6ODE
Crystal Structure of Mycobacterium tuberculosis Proteasome in Complex with Phenylimidazole-based Inhibitor B6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-07-15 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97931 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.603, 198.380, 166.513 |
| Unit cell angles | 90.00, 103.10, 90.00 |
Refinement procedure
| Resolution | 53.973 - 2.900 |
| R-factor | 0.1991 |
| Rwork | 0.197 |
| R-free | 0.24730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ts0 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.620 | 3.060 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.179 | 0.630 |
| Number of reflections | 168353 | 24489 |
| <I/σ(I)> | 6.6 | 2.1 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 3.8 | 3.8 |
| CC(1/2) | 0.973 | 0.639 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.2 | 277 | 60 mM sodium citrate, pH 6.2, 14% PEG3350 |
