6O83
S. pombe ubiquitin E1~ubiquitin-AMP tetrahedral intermediate mimic
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-11-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 122.575, 150.522, 172.776 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 113.493 - 3.153 |
R-factor | 0.2052 |
Rwork | 0.203 |
R-free | 0.25230 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4ii3 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 113.500 | 3.270 |
High resolution limit [Å] | 3.150 | 3.150 |
Rmerge | 0.113 | 0.537 |
Rmeas | 0.128 | 0.639 |
Rpim | 0.058 | 0.339 |
Number of reflections | 53605 | 4918 |
<I/σ(I)> | 10.9 | 2 |
Completeness [%] | 96.0 | 89.3 |
Redundancy | 4.2 | 3 |
CC(1/2) | 0.995 | 0.444 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 27.3% MPD, 6.6% PEG 8000, 100 mM BIS-TRIS propane pH 7.5 |