6O75
Crystal structure of Csm1-Csm4 cassette in complex with pppApA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 156.445, 156.445, 186.415 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.430 - 2.600 |
| R-factor | 0.22572 |
| Rwork | 0.223 |
| R-free | 0.27097 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6mua |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.030 | |
| Number of reflections | 41953 | 4100 |
| <I/σ(I)> | 24.3 | |
| Completeness [%] | 100.0 | |
| Redundancy | 34.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 293 | 0.1 M phosphate-citrate pH 4.2, 5% PEG3000, 25% 1,2-propanediol, and 10% glycerol |
