6O6Q
Crystal structure of Cka1p, a casein kinase 2 alpha ortholog from Candida albicans
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-08-30 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 125.690, 125.690, 229.719 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 34.598 - 2.700 |
| R-factor | 0.2361 |
| Rwork | 0.234 |
| R-free | 0.28730 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bqc |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.647 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.710 |
| High resolution limit [Å] | 2.660 | 2.660 |
| Rmerge | 0.184 | 1.004 |
| Rpim | 0.043 | 0.305 |
| Number of reflections | 31301 | 1254 |
| <I/σ(I)> | 18.57 | 1.23 |
| Completeness [%] | 98.9 | 82.1 |
| Redundancy | 18.3 | 9.3 |
| CC(1/2) | 0.787 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 2 M ammonium sulfate, 0.1 M sodium citrate pH 6.1, 1 mM AMP-PNP |
