6O42
Crystal structure of the germline PGZL1 (PGZL1_gVmDmJ) Fab in complex with MPER peptide epitope.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 208.360, 37.920, 133.520 |
| Unit cell angles | 90.00, 93.68, 90.00 |
Refinement procedure
| Resolution | 39.766 - 2.600 |
| R-factor | 0.2593 |
| Rwork | 0.257 |
| R-free | 0.31000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6o41 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.536 |
| Data reduction software | XDS (20180319) |
| Data scaling software | XSCALE (20180319) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.770 | 2.670 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rpim | 0.107 | 0.731 |
| Number of reflections | 30972 | 2317 |
| <I/σ(I)> | 6.4 | 1.2 |
| Completeness [%] | 93.8 | 95.2 |
| Redundancy | 2.9 | 2.8 |
| CC(1/2) | 0.987 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293.15 | 0.1 M sodium acetate pH 4.6, 0.2 M ammonium sulfate, 30% PEG 2000 monomethyl ether, 8 MM 06:0 PA |
