6O3P
Crystal structure of the catalytic domain of mouse Nudt12 in complex with AMP and 3 Mg2+ ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2018-02-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.207, 58.550, 61.707 |
| Unit cell angles | 102.40, 115.19, 104.66 |
Refinement procedure
| Resolution | 52.665 - 1.600 |
| R-factor | 0.181 |
| Rwork | 0.180 |
| R-free | 0.21890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5iw4 |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.350 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 52.700 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.043 | 0.653 |
| Number of reflections | 76455 | |
| <I/σ(I)> | 11.5 | |
| Completeness [%] | 89.6 | |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1 M HEPES (pH 7.5), 24% (w/v) PEG2000MME |
