6O3O
Structure of human DNAM-1 (CD226) bound to nectin-like protein-5 (necl-5)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-11-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 129.337, 182.269, 146.594 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 105.500 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rpim | 0.094 | 0.470 |
Number of reflections | 42723 | 6171 |
<I/σ(I)> | 7.9 | 1.9 |
Completeness [%] | 100.0 | 100 |
Redundancy | 19.7 | 19.8 |
CC(1/2) | 0.987 | 0.642 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350, 0.18M K2SO4, 10mM EDTA |