Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-06-30 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.11 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.685, 57.450, 86.215 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.842 - 1.700 |
R-factor | 0.1719 |
Rwork | 0.170 |
R-free | 0.20100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2anw |
RMSD bond length | 0.006 |
RMSD bond angle | 0.735 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.740 |
High resolution limit [Å] | 1.700 | 1.700 |
Rpim | 0.032 | 0.339 |
Number of reflections | 31089 | |
<I/σ(I)> | 16 | 4.1 |
Completeness [%] | 99.9 | 100 |
Redundancy | 8.9 | 9.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 24% PEG 3350, 0.2M potassium dihydrogen phosphate |