Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.127131 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.970, 121.920, 65.890 |
| Unit cell angles | 90.00, 105.79, 90.00 |
Refinement procedure
| Resolution | 28.126 - 2.200 |
| R-factor | 0.2027 |
| Rwork | 0.201 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.903 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 37739 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 88.0 | 84.1 |
| Redundancy | 2.1 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296 | 0.1 M Hepes pH 7.5 0.1 M Sodium Citrate 25 % PEG 3350 |
