6NZ7
Crystal structure of broadly neutralizing Influenza A antibody 429 B01 in complex with Hemagglutinin Hong Kong 1968
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-03-23 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 1.0000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 91.769, 123.905, 119.338 |
Unit cell angles | 90.00, 112.00, 90.00 |
Refinement procedure
Resolution | 42.225 - 2.950 |
R-factor | 0.2258 |
Rwork | 0.224 |
R-free | 0.26740 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.800 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
Rmerge | 0.105 | 0.052 | 0.509 |
Rmeas | 0.126 | 0.063 | 0.661 |
Rpim | 0.069 | 0.036 | 0.417 |
Number of reflections | 49672 | 2542 | 1543 |
<I/σ(I)> | 8.5 | ||
Completeness [%] | 94.7 | 93.8 | 59.3 |
Redundancy | 3.1 | 3 | 1.7 |
CC(1/2) | 0.993 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 300 | 10 % PEG8000 (w/v), 100 mM Tris/Cl, pH 8.5 and 7.5 % 1,6-hexanediol (w/v) |