6NPP

Crystal structure of Epstein-Barr Virus Nuclear Antigen-1, EBNA1, bound to fragments

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-01
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	59.353, 68.526, 69.897
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.621 - 1.350
R-factor	0.1588
Rwork	0.158
R-free	0.18450
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vhi
RMSD bond length	0.010
RMSD bond angle	1.027
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.370
High resolution limit [Å]	1.350	1.350
Number of reflections	61748	2490
<I/σ(I)>	16.5	1.1
Completeness [%]	97.0	80
Redundancy	9.6	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	50 mM MES pH 6.5, 0-100 mM NaCl, 10 mM DTT