6NMU
Kick-Off Fab 115 anti-SIRP-alpha antibody in complex with SIRP-alpha Variant 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-08-06 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.03319 |
| Spacegroup name | P 1 |
| Unit cell lengths | 56.931, 71.742, 77.731 |
| Unit cell angles | 98.89, 103.26, 90.07 |
Refinement procedure
| Resolution | 74.700 - 2.550 |
| R-factor | 0.1964 |
| Rwork | 0.193 |
| R-free | 0.26080 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.560 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.700 | 2.620 |
| High resolution limit [Å] | 2.550 | 2.550 |
| Number of reflections | 36050 | 3144 |
| <I/σ(I)> | 5.9 | 1.2 |
| Completeness [%] | 94.8 | 82.4 |
| Redundancy | 1.9 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.2 | 285 | 0.1 M Tris, 0.2 M MgCl2, 18% w/v PEG 4000 |
