6NK2

EphA2 LBD in complex with bA-WLA-YRPK bio peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU FR-E SUPERBRIGHT
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-10-04
Detector	RIGAKU RAXIS HTC
Wavelength(s)	1.5418
Spacegroup name	P 61
Unit cell lengths	155.441, 155.441, 51.906
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.380 - 2.200
R-factor	0.1894
Rwork	0.188
R-free	0.20920
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hei
RMSD bond length	0.003
RMSD bond angle	0.712
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	Aimless (0.6.2)
Phasing software	PHASER (2.8.2)
Refinement software	PHENIX (1.14rc2-3191)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.380	2.270
High resolution limit [Å]	2.200	2.200
Rmerge	0.156	1.438
Rmeas	0.164	1.532
Rpim	0.050	0.522
Number of reflections	36732	3157
<I/σ(I)>	13	1.6
Completeness [%]	100.0	100
Redundancy	10.8	8.5
CC(1/2)	0.998	0.527

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	293	0.09 M Sodium-Acetate pH 4.5, 22.5% w/v PEG 3,350, 3% w/v 6-aminohexanoic acid