6NK2
EphA2 LBD in complex with bA-WLA-YRPK bio peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-10-04 |
| Detector | RIGAKU RAXIS HTC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 155.441, 155.441, 51.906 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.380 - 2.200 |
| R-factor | 0.1894 |
| Rwork | 0.188 |
| R-free | 0.20920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3hei |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.712 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | Aimless (0.6.2) |
| Phasing software | PHASER (2.8.2) |
| Refinement software | PHENIX (1.14rc2-3191) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.380 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.156 | 1.438 |
| Rmeas | 0.164 | 1.532 |
| Rpim | 0.050 | 0.522 |
| Number of reflections | 36732 | 3157 |
| <I/σ(I)> | 13 | 1.6 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 | 8.5 |
| CC(1/2) | 0.998 | 0.527 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 0.09 M Sodium-Acetate pH 4.5, 22.5% w/v PEG 3,350, 3% w/v 6-aminohexanoic acid |
