6NJU

Mouse endonuclease G mutant H97A bound to A-DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-07-22
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 65 2 2
Unit cell lengths	107.983, 107.983, 357.419
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	53.992 - 2.350
R-factor	0.2256
Rwork	0.222
R-free	0.29150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5gkp
Data reduction software	XDS
Data scaling software	Aimless (v0.7.1)
Phasing software	PHASER (v2.7.16)
Refinement software	PHENIX (1.13-2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.570	2.420
High resolution limit [Å]	2.350	2.350
Rmerge	0.814	6.791
Rpim	0.256	2.231
Number of reflections	52587	4441
<I/σ(I)>	5.1
Completeness [%]	99.9	99.9
Redundancy	20.6	19.5
CC(1/2)	0.990	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	290	0.1 M sodium citrate tribasic dihydrate pH 5.5, 22% PEG 1000