6NIT

Human Argonaute2-miR-122 bound to a target RNA with four central mismatches (bu4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-06-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979500
Spacegroup name	P 21 21 21
Unit cell lengths	106.712, 137.086, 154.064
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.301 - 3.800
R-factor	0.2453
Rwork	0.243
R-free	0.29410
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.821
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.560	4.100
High resolution limit [Å]	3.790	3.790
Rmerge	0.283	1.068
Rpim	0.112	0.430
Number of reflections	23149	4605
<I/σ(I)>	5.8	1.9
Completeness [%]	98.9	97
Redundancy	7.3	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	10% PEG3350, 50mM Tris pH8, 20mM MgCl2, 75mM Phenol