6NIF
crystal structure of human REV7-RAN complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-08 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 64.571, 64.571, 113.775 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.285 - 2.002 |
R-factor | 0.2285 |
Rwork | 0.226 |
R-free | 0.26580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3abd |
RMSD bond length | 0.007 |
RMSD bond angle | 0.838 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 32.285 | 2.030 |
High resolution limit [Å] | 2.002 | 2.002 |
Rmeas | 0.086 | 0.325 |
Rpim | 0.020 | 0.076 |
Number of reflections | 18976 | 934 |
<I/σ(I)> | 41.7 | |
Completeness [%] | 99.2 | 97.16 |
Redundancy | 19.2 | 18.3 |
CC(1/2) | 0.984 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 100 mM HEPES, pH 7.0, 1.1 M sodium malonate, 0.5% Jeffamine ED-2001 |