6NFL

Crystal Structure of the Cancer Genomic DNA Mutator APOBEC3B with loop 7 from APOBEC3G complexed with 2-HP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2016-02-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	50.650, 50.650, 149.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.909 - 1.731
R-factor	0.1639
Rwork	0.162
R-free	0.19350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cqk
RMSD bond length	0.004
RMSD bond angle	0.714
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((dev_3366: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.780
High resolution limit [Å]	1.730	1.730
Rmerge	0.068	0.830
Number of reflections	21113
<I/σ(I)>	16.1	2
Completeness [%]	99.9	99.5
Redundancy	6.1	6.1
CC(1/2)		0.639

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3350