6NE7

Structure of G810A mutant of RET protein tyrosine kinase domain.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL14-1
Synchrotron site	SSRL
Beamline	BL14-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	72.286, 69.858, 78.971
Unit cell angles	90.00, 102.26, 90.00

Refinement procedure

Resolution	39.100 - 1.990
R-factor	0.20559
Rwork	0.203
R-free	0.24921
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ivt
RMSD bond length	0.008
RMSD bond angle	1.503
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.100	2.020
High resolution limit [Å]	1.990	1.990
Rmerge	0.790	0.800
Rmeas	0.950	0.973
Rpim	0.051	0.542
Number of reflections	25551	1251
<I/σ(I)>	13.66	1.3
Completeness [%]	96.6	95
Redundancy	3.3	3
CC(1/2)		0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	299	2.2-2.6M Na FORMATE, 0.1M CITRATE pH 5.5