6NDJ
Crystal structure of human NLRP6 PYD domain with MBP fusion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-02-19 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9791 |
Spacegroup name | P 1 |
Unit cell lengths | 38.283, 65.364, 102.362 |
Unit cell angles | 95.63, 92.13, 106.59 |
Refinement procedure
Resolution | 101.630 - 2.270 |
R-factor | 0.1895 |
Rwork | 0.187 |
R-free | 0.22910 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.493 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 101.630 | 2.440 |
High resolution limit [Å] | 2.270 | 2.270 |
Number of reflections | 43656 | |
<I/σ(I)> | 10.5 | |
Completeness [%] | 92.3 | |
Redundancy | 7.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.2 M lithium sulfate, 0.1 M Bis-Tris, pH 6.5, 25% PEG3350 |