6NCU

Interleukin-37 residues 53-206- dimer

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CMOS
Collection date	2016-07-09
Detector	RDI CMOS_8M
Wavelength(s)	1.000030
Spacegroup name	P 31 2 1
Unit cell lengths	67.510, 67.510, 142.740
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.103 - 3.500
R-factor	0.3463
Rwork	0.340
R-free	0.39880
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5hn1
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3228)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	54.103	3.852
High resolution limit [Å]	3.500	3.500
Rmerge	0.090	4.555
Number of reflections	5177	1382
<I/σ(I)>	16.6
Completeness [%]	99.8
Redundancy	19.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	JCSG Core I (Qiagen) screen, condition 92: 0.1M Citric acid pH 2.5, 20%MPD