6NAX

Olfactomedin domain of mouse myocilin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-BM
Synchrotron site	APS
Beamline	22-BM
Temperature [K]	80
Detector technology	CCD
Collection date	2017-02-11
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 4
Unit cell lengths	111.955, 111.955, 44.129
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.100 - 1.551
R-factor	0.1582
Rwork	0.158
R-free	0.17790
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4wxq
RMSD bond length	0.008
RMSD bond angle	0.873
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX ((1.11.1_2575))
Refinement software	PHENIX ((1.11.1_2575))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.100	1.607
High resolution limit [Å]	1.550	1.551
Rmerge	0.067	0.210
Rmeas	0.076	0.242
Rpim	0.035	0.118
Number of reflections	79283	7499
<I/σ(I)>	18.04	6.06
Completeness [%]	98.5	95.2
Redundancy	4.5	3.9
CC(1/2)	0.996	0.950

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289	1:1 drop ratio of 30 mg/mL mouse OLF in 10 mM Na/K Phosphate pH 7.2 and mother liquor comprising 10% PEG 8000, 200 mM magnesium chloride