6N9O
Crystal structure of human GSDMD
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.640, 105.550, 151.790 |
| Unit cell angles | 90.00, 94.47, 90.00 |
Refinement procedure
| Resolution | 49.483 - 3.500 |
| R-factor | 0.2796 |
| Rwork | 0.276 |
| R-free | 0.34540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b5r 6ao3 6ao4 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.891 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX ((1.14_3260: ???)) |
| Refinement software | PHENIX ((1.15.2_3472: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 3.600 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmeas | 0.086 | |
| Number of reflections | 23251 | 1695 |
| <I/σ(I)> | 10.2 | 1.4 |
| Completeness [%] | 99.1 | 99.3 |
| Redundancy | 3.2 | |
| CC(1/2) | 0.997 | 0.605 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 25% PEG3350, 0.1M Bis-Tris pH 5.5, 200 mM NaCl, and 10 mM DTT |
