6N8W
Structure of Unliganded Hsp90-Beta N-Terminal Domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-02-13 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.03320 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 175.250, 70.249, 101.606 |
| Unit cell angles | 90.00, 123.45, 90.00 |
Refinement procedure
| Resolution | 28.886 - 3.092 |
| R-factor | 0.231030896435 |
| Rwork | 0.225 |
| R-free | 0.28588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.510 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.200 | 3.310 |
| High resolution limit [Å] | 3.090 | 3.090 |
| Rmerge | 0.094 | 0.417 |
| Number of reflections | 18636 | 3063 |
| <I/σ(I)> | 8.1 | 1.8 |
| Completeness [%] | 97.6 | 89 |
| Redundancy | 2.5 | 2.4 |
| CC(1/2) | 0.991 | 0.686 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | Tris-HCl pH 8.5, MgCl2, PEG 4000, Glycerol |
