6N8S
Crystal structure of the human cell polarity protein Lethal Giant Larvae 2 (Lgl2). aPKC phosphorylated, crystal form 3.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 130.871, 130.871, 277.649 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.660 - 3.900 |
| R-factor | 0.2556 |
| Rwork | 0.252 |
| R-free | 0.29780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.453 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.660 | 4.210 |
| High resolution limit [Å] | 3.900 | 3.900 |
| Rmerge | 1.246 | 4.841 |
| Number of reflections | 22815 | 4624 |
| <I/σ(I)> | 4 | 1.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 13.1 | 13.8 |
| CC(1/2) | 0.953 | 0.416 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295.15 | 1.4 M ammonium sulfate, 100 mM Hepes pH 7.5, 150 mM NaCl, 1.07% 1,6-hexanediol |
