6N8P

Crystal structure of the human cell polarity protein Lethal Giant Larvae 2 (Lgl2). Unphosphorylated, crystal form 1.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-07-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0055
Spacegroup name	P 21 21 21
Unit cell lengths	78.699, 118.489, 126.639
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.349 - 3.193
R-factor	0.2081
Rwork	0.204
R-free	0.24970
RMSD bond length	0.002
RMSD bond angle	0.479
Data reduction software	XDS
Data scaling software	Aimless
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.350	3.410
High resolution limit [Å]	3.190	3.190
Rmerge	0.419	2.097
Number of reflections	20282	3625
<I/σ(I)>	5.9	1.7
Completeness [%]	99.8	99.5
Redundancy	6.5	6.8
CC(1/2)	0.972	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295.15	19.8 % PEG 3350, 0.29 M Na2SO4, 0.1 M bis-tris propane pH 7.5, 3% methanol