6N4Y

Metabotropic Glutamate Receptor 5 Extracellular Domain with Nb43

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	95.528, 158.389, 111.963
Unit cell angles	90.00, 101.93, 90.00

Refinement procedure

Resolution	38.870 - 3.262
R-factor	0.2014
Rwork	0.199
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lmk
Data reduction software	XDS
Data scaling software	Aimless (0.5.25)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3211)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	39.600	39.600	3.370
High resolution limit [Å]	3.260	13.050	3.260
Rmerge	0.132	0.046	0.819
Rmeas	0.156	0.056	0.969
Rpim	0.083	0.032	0.512
Total number of observations	174911	2497	15021
Number of reflections	49878	778	4389
<I/σ(I)>	7.6	22.7	1.4
Completeness [%]	98.4	94.2	94
Redundancy	3.5	3.2	3.4
CC(1/2)	0.926	0.982	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	18-20% PEG 3350 0.15M Potassium Nitrate 1% Benzamidine