6N4X
Metabotropic Glutamate Receptor 5 Apo Form Ligand Binding Domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 78 |
Detector technology | PIXEL |
Collection date | 2014-06-07 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 2 2 21 |
Unit cell lengths | 119.084, 174.100, 180.902 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.569 - 4.000 |
R-factor | 0.2706 |
Rwork | 0.270 |
R-free | 0.28210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6n4y |
Data reduction software | XDS |
Data scaling software | Aimless (0.1.26) |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3211) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.570 | 49.570 | 4.220 |
High resolution limit [Å] | 4.000 | 12.650 | 4.000 |
Rmerge | 0.140 | 0.054 | 1.671 |
Rmeas | 0.151 | 0.057 | 1.808 |
Rpim | 0.054 | 0.020 | 0.667 |
Number of reflections | 30328 | 997 | 4390 |
<I/σ(I)> | 11.2 | ||
Completeness [%] | 94.0 | 89.1 | 94.2 |
Redundancy | 6.9 | 6.4 | 6.7 |
CC(1/2) | 0.998 | 0.998 | 0.470 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 15% PEG 3350, 0.2M Na Cacodylate pH 6.5 |