6N3D

Structure of HIV Tat-specific factor 1 U2AF Homology Motif (APO-State)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-19
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.8265
Spacegroup name	P 43
Unit cell lengths	30.201, 30.201, 98.742
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.200 - 1.130
R-factor	0.1285
Rwork	0.127
R-free	0.15960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fxw 2peh 4oz0
Data reduction software	XDS
Data scaling software	Aimless (0.1.26)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.200	1.170
High resolution limit [Å]	1.130	1.130
Rmerge	0.054	0.252
Rpim	0.033	0.252
Number of reflections	61046	1696
<I/σ(I)>	13.6	2.9
Completeness [%]	98.8	99.6
Redundancy	8.7	1.9
CC(1/2)	0.990	0.840

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277.15	15% PEG 8000, 0.4 M MgCl2, 0.1 M TRIS