6MZ3

mCherry pH sensitive mutant - M66T (mCherryTYG)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2017-03-08
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.088
Spacegroup name	P 1 21 1
Unit cell lengths	48.806, 42.782, 61.067
Unit cell angles	90.00, 112.20, 90.00

Refinement procedure

Resolution	19.979 - 1.088
R-factor	0.1671
Rwork	0.167
R-free	0.17760
RMSD bond length	0.005
RMSD bond angle	0.992
Data reduction software	XDS
Data scaling software	Aimless (0.5.31)
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.979	1.210
High resolution limit [Å]	1.088	1.090
Number of reflections	76260
<I/σ(I)>	10.4
Completeness [%]	93.6
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292	50 mM Tris-HCl, 100 mM NaOAc, 30% PEG 4000, pH 8.5