6MYT

Avian mitochondrial complex II with Atpenin A5 bound, sidechain in pocket

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	69.992, 83.817, 290.344
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.190 - 2.270
R-factor	0.227
Rwork	0.226
R-free	0.26000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1yq3
RMSD bond length	0.002
RMSD bond angle	0.533
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX ((DEV_3150: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.230	2.340
High resolution limit [Å]	2.270	2.270
Rmerge	0.145	0.557
Number of reflections	70243
<I/σ(I)>	4.1	1.5
Completeness [%]	87.6	67.8
Redundancy	2.15	1.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	47 g/L PEG3350, 30 mL/L PEG400, 23 mL/L isopropanol, 0.05 M HEPES sodium, 0.01 M Tris-HCl, 3.1 mM manganese chloride, 0.62 mM magnesium chloride, 1.5 mM sodium azide, 0.25 mM sodium EDTA, 10 g/L octyl beta-D-glucoside, undecyl-beta-D-maltoside, crystal was soaked with Atpenin A5 after crystallization