6MYJ

Pleurotus ostreatus OstreolysinA plus sphingomyelin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97926
Spacegroup name	P 1 21 1
Unit cell lengths	46.425, 100.565, 58.813
Unit cell angles	90.00, 106.29, 90.00

Refinement procedure

Resolution	44.561 - 1.330
R-factor	0.1548
Rwork	0.155
R-free	0.16750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ov8
RMSD bond length	0.016
RMSD bond angle	0.949
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.350
High resolution limit [Å]	1.330	3.610	1.330
Rmerge	0.046	0.028	0.429
Rmeas	0.049	0.030	0.462
Rpim	0.018	0.011	0.170
Number of reflections	117866	6004	5878
<I/σ(I)>	8
Completeness [%]	99.4	99.5	99.7
Redundancy	7.5	7.7	7.2
CC(1/2)		0.999	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M ammonium chloride, 0.05 M tris hydrochloride, 0.15 M sodium chloride, 19% (w/v) PEG3350, saturated sphingomyelin, saturated cholesterol, 30% ethylene glycol