6MXC
Crystal structure of Trypanosoma brucei hypoxanthine-guanine-xanthine phosphoribosyltranferase in complex with GMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX1 |
Synchrotron site | Australian Synchrotron |
Beamline | MX1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-04-06 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 61.021, 107.321, 117.214 |
Unit cell angles | 90.00, 96.93, 90.00 |
Refinement procedure
Resolution | 44.744 - 1.993 |
R-factor | 0.1533 |
Rwork | 0.152 |
R-free | 0.19970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6AP2 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.151 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.744 | 2.030 |
High resolution limit [Å] | 1.990 | 1.990 |
Rmerge | 0.088 | |
Rpim | 0.061 | 0.213 |
Number of reflections | 102156 | 4961 |
<I/σ(I)> | 11.3 | 3 |
Completeness [%] | 99.9 | 98.7 |
Redundancy | 5.7 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293.15 | 25% PEG 3350, 0.2 M lithium sulphate and 0.1 M Bis-Tris pH 5 |