6MW7
Crystal structure of ATPase module of SMCHD1 bound to ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 173 |
| Detector technology | PIXEL |
| Collection date | 2018-07-05 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 108.756, 148.012, 191.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.785 - 2.194 |
| R-factor | 0.1982 |
| Rwork | 0.197 |
| R-free | 0.23010 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.664 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((1.10.1_2155)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.240 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rpim | 0.039 | 0.287 |
| Number of reflections | 153427 | 6560 |
| <I/σ(I)> | 4.1 | 1.2 |
| Completeness [%] | 97.4 | 84.2 |
| Redundancy | 9.1 | 7 |
| CC(1/2) | 0.841 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 25 mM Bis Tris Propane pH 6.8, 50mM Ammonium sulfate, 5% glycerol, 7.5% PEG 8000, 5mM DTT |
