6MVM
LasR LBD L130F:3OC14HSL complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2017-12-07 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.881, 70.774, 53.817 |
| Unit cell angles | 90.00, 90.15, 90.00 |
Refinement procedure
| Resolution | 29.568 - 1.895 |
| R-factor | 0.2231 |
| Rwork | 0.213 |
| R-free | 0.26540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uv0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.021 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.980 |
| High resolution limit [Å] | 1.895 | 1.900 |
| Number of reflections | 25887 | |
| <I/σ(I)> | 14.4 | |
| Completeness [%] | 96.6 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 200 mM magnesium nitrate, 20% PEG3350 |
