6MV7

Crystal structure of RNAse 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-10-04
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	27.650, 38.830, 97.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.095 - 2.590
R-factor	0.2204
Rwork	0.217
R-free	0.25190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6mv6
RMSD bond length	0.003
RMSD bond angle	0.703
Data reduction software	HKL-2000
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.100	2.710
High resolution limit [Å]	2.590	2.600
Number of reflections	3537	308
<I/σ(I)>	17.2
Completeness [%]	98.6
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		298	PEG 4000, 50mM Sodium phosphate