6MV5
Anti-PCSK9 fab 6E2 bound to the N-terminal peptide from PCSK9 (E32K)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-18 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 70.449, 100.420, 86.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.000 - 2.100 |
R-factor | 0.2107 |
Rwork | 0.210 |
R-free | 0.23850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6e4y |
RMSD bond length | 0.004 |
RMSD bond angle | 0.958 |
Data reduction software | MOSFLM |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.000 | 2.160 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.073 | 0.759 |
Rmeas | 0.089 | 0.929 |
Rpim | 0.050 | |
Number of reflections | 36442 | 3626 |
<I/σ(I)> | 10.7 | 2 |
Completeness [%] | 99.4 | 98.9 |
Redundancy | 5.5 | 5.6 |
CC(1/2) | 0.995 | 0.627 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 15% PEG8000, 0.2 M zinc acetate, 0.1 M sodium cacodylate, pH 6.5 |