6MUD
Voltage-gated sodium channel NaV1.5 C-terminal domain in complex with Ca2+/Calmodulin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-09-17 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033000 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 65.579, 84.032, 140.684 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.310 - 2.690 |
| R-factor | 0.2147 |
| Rwork | 0.212 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.055 |
| Data reduction software | XDS (November 11, 2013) |
| Data scaling software | XDS (November 11, 2013) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0222) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.850 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmeas | 0.097 | 0.494 |
| Number of reflections | 10476 | 1471 |
| <I/σ(I)> | 9.51 | 1.89 |
| Completeness [%] | 93.9 | 83.3 |
| Redundancy | 3.25 | 2.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 277 | 5-15 % (w/v) PEG 4000, 0.1 M Tris, pH 9.5, 0.1 M MgCl2, and 5 % (v/v) isopropanol |
