6MU4

Bst DNA polymerase I FANA/DNA binary complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-21
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	87.835, 93.898, 105.809
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.092 - 1.620
R-factor	0.1878
Rwork	0.187
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1l3s
RMSD bond length	0.006
RMSD bond angle	0.936
Data reduction software	autoXDS
Data scaling software	autoXDS
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.092	1.670
High resolution limit [Å]	1.620	1.620
Rmerge	0.041	0.846
Number of reflections	111377
<I/σ(I)>	23.32	2.22
Completeness [%]	99.7
Redundancy	6.6
CC(1/2)	1.000	0.824

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	50% ammonium sulfate, 0.1M MES, 2% MPD