6MR4
Crystal structure of the Sth1 bromodomain from S.cerevisiae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97918 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 141.753, 74.619, 72.541 |
| Unit cell angles | 90.00, 92.88, 90.00 |
Refinement procedure
| Resolution | 72.449 - 2.710 |
| R-factor | 0.2394 |
| Rwork | 0.238 |
| R-free | 0.26990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3tlp |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.610 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.450 | 2.840 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Rmerge | 0.083 | |
| Number of reflections | 20385 | 2722 |
| <I/σ(I)> | 10.8 | 1.7 |
| Completeness [%] | 98.6 | 99.5 |
| Redundancy | 3.1 | 3.2 |
| CC(1/2) | 0.990 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M magnesium chloride hexahydrate 0.1 M BIS-TRIS pH 6.5 25% (w/v) Polyethylene glycol 3350 3% Trimethylamine N-oxide dihydrate |
