6MQT
HRAS G12S in complex with GDP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-12 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 122.808, 79.773, 134.062 |
| Unit cell angles | 90.00, 101.94, 90.00 |
Refinement procedure
| Resolution | 39.445 - 1.498 |
| R-factor | 0.1591 |
| Rwork | 0.157 |
| R-free | 0.19520 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b2z |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (dev_2932) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.500 |
| Rpim | 0.025 | 95.500 |
| Number of reflections | 195200 | 9667 |
| <I/σ(I)> | 39.7 | 6.39 |
| Completeness [%] | 96.6 | 95.5 |
| Redundancy | 6.7 | |
| CC(1/2) | 0.990 | 0.987 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.2 M Sodium acetate trihydrate, 0.1 M TRIS hydrochloride pH 8.5, 30% w/v Polyethylene glycol 4,000 |
