6MQL
Crystal Structure of GTPase Domain of Human Septin 12 Mutant T89M
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-10-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 83.920, 56.490, 70.290 |
Unit cell angles | 90.00, 114.86, 90.00 |
Refinement procedure
Resolution | 28.245 - 2.170 |
R-factor | 0.2152 |
Rwork | 0.214 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6mq9 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.558 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.250 | 2.230 |
High resolution limit [Å] | 2.170 | 2.170 |
Rpim | 0.059 | 0.488 |
Number of reflections | 15878 | 1161 |
<I/σ(I)> | 8.7 | 1.7 |
Completeness [%] | 99.6 | 99.6 |
Redundancy | 6.5 | 6.7 |
CC(1/2) | 0.995 | 0.528 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 10% w/v PEG 4000, 20% v/v glycerol, 0.1 M bicine/Trizma base pH 8.5, 30mM of each: diethyleneglycol, triethyleneglycol, tetraethyleneglycol, pentaethyleneglycol |