6MQ8

Binary structure of DNA polymerase eta in complex with templating hypoxanthine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-23
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0
Spacegroup name	P 61
Unit cell lengths	98.539, 98.539, 81.664
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.270 - 1.969
R-factor	0.1732
Rwork	0.171
R-free	0.20760
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.000
High resolution limit [Å]	1.970	5.350	1.970
Rmerge	0.083	0.043	0.534
Rmeas	0.093	0.048	0.596
Rpim	0.040	0.020	0.257
Total number of observations	162166
Number of reflections	31977	1652	1580
<I/σ(I)>	5.9
Completeness [%]	99.9	99.8	100
Redundancy	5.1	5.1	5.1
CC(1/2)		0.998	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	291.15	100mM MES 5.5, 10% PEG 2K MME, 5mM MgSO4