6MOL
Monoextended DARPin M_R12 complex with EpoR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033202 |
| Spacegroup name | P 63 |
| Unit cell lengths | 168.109, 168.109, 78.029 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.969 - 3.163 |
| R-factor | 0.2232 |
| Rwork | 0.219 |
| R-free | 0.25990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | predicted model 1ern |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.494 |
| Data reduction software | XDS (Jan 26, 2018) |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.969 | 3.400 |
| High resolution limit [Å] | 3.163 | 3.163 |
| Rmerge | 0.198 | 2.046 |
| Rpim | 0.070 | 0.832 |
| Number of reflections | 14501 | 724 |
| <I/σ(I)> | 6.22 | 0.24 |
| Completeness [%] | 66.6 | 17.3 |
| Redundancy | 8.7 | 6.8 |
| CC(1/2) | 0.995 | 0.491 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 295 | Seeded from 0.2 M NaCl, 0.1 M CAPS pH 10.5, 20% PEG 8000 into 0.2 M ammonium tartrate and 20% PEG 3350, with 10% glycerol, 10% ethylene glycol as cryoprotectant |
