6MOL

Monoextended DARPin M_R12 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-07-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.033202
Spacegroup name	P 63
Unit cell lengths	168.109, 168.109, 78.029
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	44.969 - 3.163
R-factor	0.2232
Rwork	0.219
R-free	0.25990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	predicted model 1ern
RMSD bond length	0.002
RMSD bond angle	0.494
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3211: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.969	3.400
High resolution limit [Å]	3.163	3.163
Rmerge	0.198	2.046
Rpim	0.070	0.832
Number of reflections	14501	724
<I/σ(I)>	6.22	0.24
Completeness [%]	66.6	17.3
Redundancy	8.7	6.8
CC(1/2)	0.995	0.491

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	Seeded from 0.2 M NaCl, 0.1 M CAPS pH 10.5, 20% PEG 8000 into 0.2 M ammonium tartrate and 20% PEG 3350, with 10% glycerol, 10% ethylene glycol as cryoprotectant