6MOF

Monomeric DARPin G2 complex with EpoR

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-23
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 41 21 2
Unit cell lengths	108.834, 108.834, 96.264
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.053 - 2.894
R-factor	0.2279
Rwork	0.224
R-free	0.26570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ern predicted model of G2
RMSD bond length	0.001
RMSD bond angle	0.441
Data reduction software	XDS (Jun 1, 2017)
Data scaling software	XDS (Jun 1, 2017)
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.053	2.998
High resolution limit [Å]	2.894	2.894
Rmerge	0.309	1.172
Rpim	0.089	0.353
Number of reflections	13260	1269
<I/σ(I)>	18.83	1.18
Completeness [%]	98.7	96.87
Redundancy	12.2	11.7
CC(1/2)	0.999	0.375

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	295	0.2 M Magnesium chloride, 0.1 M Tris pH 7.0, 10% PEG 8000, 25% ethylene glycol as cryoprotectant