6ML1
Structure of the USP15 deubiquitinase domain in complex with an affinity-matured inhibitory Ubv
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-04 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 42.770, 115.290, 95.320 |
Unit cell angles | 90.00, 91.92, 90.00 |
Refinement procedure
Resolution | 49.319 - 1.900 |
R-factor | 0.1909 |
Rwork | 0.189 |
R-free | 0.21950 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | USP15 and Ubv from 6CRN |
RMSD bond length | 0.007 |
RMSD bond angle | 0.817 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 95.270 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.031 | 0.317 |
Number of reflections | 71233 | 3864 |
<I/σ(I)> | 15.5 | 2.9 |
Completeness [%] | 98.1 | 81.6 |
Redundancy | 3.9 | 3.2 |
CC(1/2) | 0.998 | 0.987 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 295 | 16% PEG3350, 100 mM MES 6.0, 250 mM CaCl2, cryoprotected with 18% PEG3350, 200 mM CaCl2, 100 mM MES pH 6.5 and 25% glycerol |