6MIM

Crystal structure of AF9 YEATS domain Y78W mutant in complex with histone H3K9cr

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2017-08-17
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	42.159, 59.697, 155.395
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.338 - 2.525
R-factor	0.209
Rwork	0.203
R-free	0.26370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4tmp
RMSD bond length	0.012
RMSD bond angle	1.353
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.620
High resolution limit [Å]	2.520	2.530
Number of reflections	13056
<I/σ(I)>	33.5
Completeness [%]	94.3
Redundancy	10.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	100 mM MES pH 6.0, 20%(w/v) PEG 6000, and 10 mM ZnCl2