6MHF
Galphai3 co-crystallized with GIV/Girdin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-09-08 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.99997 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 83.780, 83.780, 141.370 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 54.030 - 2.000 |
R-factor | 0.2078 |
Rwork | 0.206 |
R-free | 0.23910 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4g5r |
RMSD bond length | 0.002 |
RMSD bond angle | 0.526 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.080 | 2.080 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 63761 | 34165 |
<I/σ(I)> | 29.5 | |
Completeness [%] | 93.9 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | PEG 3350, ammonium chloride |