6MHE

Galphai3 co-crystallized with KB752

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2016-06-16
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99999
Spacegroup name	P 43 21 2
Unit cell lengths	83.240, 83.240, 133.830
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	52.150 - 2.200
R-factor	0.2299
Rwork	0.228
R-free	0.26400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4g5r
RMSD bond length	0.002
RMSD bond angle	0.497
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.910	2.270
High resolution limit [Å]	2.200	2.200
Number of reflections	44127	24419
<I/σ(I)>	15.91
Completeness [%]	94.0
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277.15	PEG 3350, ammonium chloride