6MGM
Helix-Loop-helix motif of mouse DNA-binding protein inhibitor ID-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-16 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.95 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.165, 28.979, 50.333 |
| Unit cell angles | 90.00, 97.94, 90.00 |
Refinement procedure
| Resolution | 21.437 - 1.791 |
| R-factor | 0.1832 |
| Rwork | 0.178 |
| R-free | 0.22270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mdy |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.727 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rpim | 0.044 | 0.231 |
| Number of reflections | 8782 | |
| <I/σ(I)> | 32.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | 0.1 M sodium citrate (pH 6.5), 0.2 M magnesium acetate, 10% PEG8000 |
