6MG1

C-terminal bZIP domain of human C/EBPbeta with 16bp Methylated Oligonucleotide Containing Consensus Recognition Sequence-C2 Crystal Form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	RIGAKU MICROMAX-003
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-02-04
Detector	RIGAKU HyPix-6000HE
Wavelength(s)	1.00000
Spacegroup name	C 1 2 1
Unit cell lengths	48.110, 77.405, 99.825
Unit cell angles	90.00, 103.90, 90.00

Refinement procedure

Resolution	34.542 - 1.750
R-factor	0.2156
Rwork	0.214
R-free	0.24960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e42
RMSD bond length	0.005
RMSD bond angle	0.996
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.542	1.690
High resolution limit [Å]	1.630	1.630
Rmerge	0.080	0.519
Rpim	0.024	0.374
Number of reflections	35611	842
<I/σ(I)>	25.4	1.2
Completeness [%]	80.8
Redundancy	10.3
CC(1/2)		0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	292	0.2 M sodium chloride, 0.1 M BIS-TRIS pH 6.5, 25% (w/v) polyethylene glycol 3350